Solid-Phase Peptide Synthesis (SPPS)
Solid-Phase Peptide Synthesis (SPPS), developed by Robert Bruce Merrifield (Nobel Prize, 1984), is the standard method for producing synthetic peptides. The process anchors the C-terminal amino acid to an insoluble polymer resin, then sequentially adds amino acids from C-terminus to N-terminus.
Modern SPPS uses Fmoc (fluorenylmethyloxycarbonyl) chemistry. Each cycle involves: (1) Fmoc deprotection with piperidine, (2) coupling the next amino acid using activating reagents, (3) washing to remove excess reagents. After chain assembly, the peptide is cleaved from the resin and side-chain protecting groups are removed.
The crude peptide is then purified by HPLC, analyzed by mass spectrometry, and lyophilized.
All Healthy Aminos products are for research use only.
Related Products
Related Terms
Peptide Bond
A covalent chemical bond formed between the carboxyl group of one amino acid and the amino group of another through a condensation reaction, linking amino acids into chains.
HPLC
High-Performance Liquid Chromatography — the standard analytical technique for determining peptide purity by separating molecular species based on their chemical properties.
Lyophilization
Freeze-drying — a dehydration process that removes water from a frozen peptide solution under vacuum, producing a stable dry powder.
Amino Acid Sequence
The linear order of amino acid residues in a peptide or protein chain, written from N-terminus to C-terminus, which determines the molecule's identity and properties.
Research Use Only
FOR RESEARCH USE ONLY. Products sold by Healthy Aminos are intended strictly for in-vitro research and laboratory use. Not for human or animal consumption. Not FDA approved. By purchasing from Healthy Aminos, the buyer acknowledges that these products are not intended to diagnose, treat, cure, or prevent any disease. All products are sold as reference standards and research chemicals only.