Reconstitution
Reconstitution is the process of adding a solvent to a lyophilized peptide powder to create a solution for research use. Common solvents include sterile water, bacteriostatic water (containing 0.9% benzyl alcohol as a preservative), or buffered saline.
The solvent is typically added slowly down the inside wall of the vial, then gently swirled — not shaken — to dissolve the powder. Vigorous agitation can cause foaming and protein aggregation. The reconstituted solution should be clear; turbidity may indicate aggregation or insolubility.
Once reconstituted, peptide solutions are significantly less stable than lyophilized powders and should be stored at 2-8°C for short-term use or aliquoted and frozen at -20°C.
All Healthy Aminos products are for research use only.
Related Products
Related Terms
Lyophilization
Freeze-drying — a dehydration process that removes water from a frozen peptide solution under vacuum, producing a stable dry powder.
Bacteriostatic Water
Sterile water containing 0.9% benzyl alcohol as a bacteriostatic preservative, commonly used for reconstituting peptides.
Aliquoting
Dividing a reconstituted peptide solution into smaller portions for individual use, minimizing freeze-thaw cycles on the bulk solution.
Shelf Life
The duration a peptide product maintains acceptable quality when stored under specified conditions.
Research Use Only
FOR RESEARCH USE ONLY. Products sold by Healthy Aminos are intended strictly for in-vitro research and laboratory use. Not for human or animal consumption. Not FDA approved. By purchasing from Healthy Aminos, the buyer acknowledges that these products are not intended to diagnose, treat, cure, or prevent any disease. All products are sold as reference standards and research chemicals only.