Net Peptide Content
Net peptide content (NPC) measures the fraction of the total powder weight in a vial that is actual peptide material. The remainder consists of water (typically 5-15%), TFA counter-ions from synthesis (10-20%), and trace residual solvents.
NPC is distinct from HPLC purity. A vial labeled "5mg, ≥99% pure" might have an NPC of 80%, meaning: 5.0mg gross × 0.80 NPC × 0.99 purity = 3.96mg actual target peptide. This is normal — NPC values of 60-85% are standard for lyophilized peptides.
NPC is determined by amino acid analysis, nitrogen analysis, or UV spectrophotometry. Researchers using precise concentrations should account for NPC when calculating working solutions.
All Healthy Aminos products are for research use only.
Related Terms
Purity Percentage
The proportion of target peptide relative to total detected material in an HPLC analysis, expressed as a percentage of chromatographic peak area.
TFA Salt
Trifluoroacetic acid salt — a counter-ion remaining from HPLC purification that contributes to the non-peptide mass in lyophilized preparations.
Amino Acid Analysis
An analytical technique that hydrolyzes a peptide and quantifies individual amino acid residues, confirming sequence composition.
Lyophilization
Freeze-drying — a dehydration process that removes water from a frozen peptide solution under vacuum, producing a stable dry powder.
Research Use Only
FOR RESEARCH USE ONLY. Products sold by Healthy Aminos are intended strictly for in-vitro research and laboratory use. Not for human or animal consumption. Not FDA approved. By purchasing from Healthy Aminos, the buyer acknowledges that these products are not intended to diagnose, treat, cure, or prevent any disease. All products are sold as reference standards and research chemicals only.