HPLC
High-Performance Liquid Chromatography (HPLC) is the gold standard method for determining the purity of synthetic peptides. In reverse-phase HPLC (RP-HPLC), a sample is dissolved and injected into a C18 column under high pressure. Molecules separate based on hydrophobicity — more hydrophobic species elute later.
UV detection at 214-220nm measures the absorbance of each eluting species. The result is a chromatogram showing peaks corresponding to different molecular components. The target peptide should appear as the dominant peak. Purity is calculated as: (target peak area / total peak area) × 100.
Research-grade peptides should achieve ≥98% HPLC purity. Method parameters — column type, mobile phase, gradient program, flow rate — should be documented on the Certificate of Analysis for reproducibility.
All Healthy Aminos products are for research use only.
Related Products
Related Terms
Chromatogram
A graphical output from HPLC analysis showing UV absorbance vs retention time, where peaks represent different molecular species in the sample.
Purity Percentage
The proportion of target peptide relative to total detected material in an HPLC analysis, expressed as a percentage of chromatographic peak area.
Certificate of Analysis (COA)
A document issued by an analytical laboratory reporting the results of identity, purity, and safety testing performed on a specific batch of material.
Mass Spectrometry
An analytical technique that measures molecular weight by ionizing molecules and separating them by mass-to-charge ratio, used to confirm peptide identity.
Research Use Only
FOR RESEARCH USE ONLY. Products sold by Healthy Aminos are intended strictly for in-vitro research and laboratory use. Not for human or animal consumption. Not FDA approved. By purchasing from Healthy Aminos, the buyer acknowledges that these products are not intended to diagnose, treat, cure, or prevent any disease. All products are sold as reference standards and research chemicals only.