Amidation
Amidation is a chemical modification that replaces the free carboxyl group (-COOH) at a peptide's C-terminus with an amide group (-CONH2). This neutralizes the negative charge of the terminal carboxylate.
C-terminal amidation is widespread in bioactive peptides. Ipamorelin (Aib-His-D-2Nal-D-Phe-Lys-NH2) and MT-II both feature C-terminal amide groups. Many naturally occurring neuropeptides and hormones are also C-terminally amidated.
Amidation can increase resistance to carboxypeptidase degradation and may influence receptor binding. In solid-phase peptide synthesis, amidated peptides are produced using Rink amide resin, which releases the peptide with a C-terminal amide upon cleavage.
All Healthy Aminos products are for research use only.
Related Products
Related Terms
C-Terminus
The end of a peptide chain with a free carboxyl group (-COOH), conventionally written as the last residue in a sequence.
Acetylation
The addition of an acetyl group (CH3CO-) to the N-terminus of a peptide, commonly used to increase metabolic stability.
Cyclization
The formation of a ring structure within a peptide, typically through side-chain-to-side-chain, head-to-tail, or disulfide linkages, often increasing metabolic stability.
Research Use Only
FOR RESEARCH USE ONLY. Products sold by Healthy Aminos are intended strictly for in-vitro research and laboratory use. Not for human or animal consumption. Not FDA approved. By purchasing from Healthy Aminos, the buyer acknowledges that these products are not intended to diagnose, treat, cure, or prevent any disease. All products are sold as reference standards and research chemicals only.