Compound Profiles
TB-500 (Thymosin Beta-4): Published Research on a Regenerative Peptide
Healthy Aminos Research Team · · 6 min read
Molecular Profile
TB-500 is a synthetic analog of Thymosin Beta-4 (Tβ4), a 43-amino acid peptide naturally present in most mammalian tissues and cell types. Thymosin Beta-4 was first isolated from calf thymus tissue in the 1960s by Dr. Allan Goldstein at the National Institutes of Health. TB-500's molecular weight is approximately 4921 Da (CAS: 77591-33-4).
The peptide's primary molecular function involves interaction with G-actin (globular actin), regulating actin polymerization — a process fundamental to cell motility, migration, and cytoskeletal organization. The active domain responsible for actin binding is the LKKTET sequence at amino acids 17-22.
Published Research
### Wound Healing Studies
In a 1999 study, [Malinda et al.](https://pubmed.ncbi.nlm.nih.gov/10469335/) examined Thymosin Beta-4 in dermal wound models. The researchers reported 42-61% increases in re-epithelialization rates in treated groups compared to controls, along with increased angiogenesis at wound sites. The study used topical application in a rat full-thickness skin wound model.
### Multi-Functional Review
[Goldstein et al. (2012)](https://pubmed.ncbi.nlm.nih.gov/22074294/) published a comprehensive review of Thymosin Beta-4 research spanning cardiac repair, wound healing, corneal healing, and neurological models. The review documented published findings across multiple species and tissue types, noting the peptide's observed effects on cell migration, angiogenesis, and inflammatory markers in various preclinical systems.
### Corneal Healing and Clinical Data
[Kleinman & Sosne (2012)](https://pubmed.ncbi.nlm.nih.gov/23050815/) reviewed both preclinical data and early phase 2 clinical trial results for Thymosin Beta-4 in corneal wound healing. This represents some of the limited human clinical data available for TB-500-related compounds, with the authors reporting observations on corneal epithelial healing rates in treated versus placebo groups.
Research Considerations
TB-500 has a broader base of published research than many research peptides, including some human clinical data in corneal models. However, most published studies remain preclinical. The compound is not FDA-approved for any indication.
Research-grade [TB-500](/peptides/healing/tb-500) is supplied as a lyophilized powder with ≥98% HPLC purity. Store at -20°C for long-term stability.
*All Healthy Aminos products are for research use only. Not for human or animal consumption. Not FDA approved.*
Research Use Only
FOR RESEARCH USE ONLY. Products sold by Healthy Aminos are intended strictly for in-vitro research and laboratory use. Not for human or animal consumption. Not FDA approved. By purchasing from Healthy Aminos, the buyer acknowledges that these products are not intended to diagnose, treat, cure, or prevent any disease. All products are sold as reference standards and research chemicals only.